Assignment:
Plotting RSA versus Protein Percentage
1. Subcellular fractionation of rat liver resulted in the following samples:
Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml)
P-1............2800..................10........................6
P-2............12000..................10......................4
P-3............27000..................8........................4
P-4............75000..................5........................2.5
S............non-sedimentable..25....................6.5
2. Analysis of the fractions resulted in the following enzyme activities expressed as
nmoles/min/ml of sample fraction.
Fraction......citrate synthase......catalase......phosphatase......aldolase
P-1..................0.20........................0.10............0.80..................1.0
P-2..................8.00........................1.00............9.00..................2.0
P-3..................6.00........................7.00............14.0..................1.8
P-4..................0.50........................17.5............12.0..................0.9
S.....................0.04........................0.40............0.70..................15.0
[RSA
Relative Specific
Activity ]..................P-1............ P-2............ P-3............ P-4............ S
Citrate synthase...0.033..........2.00...........1.50............0.20........ 0.006
Catalase...............0.016..........0.25...........1.75............7.00.........0.061
Phosphatase........0.13............2.25............3.50............4.80........0.107
Aldolase...............0.16............0.50............0.45............0.36.........2.30
Plot the distribution profile for each enzyme as RSA vs. % protein