PROTEIN FOLDING ASSIGNMENT
In an "in vitro" protein folding experiment monitored by fluorescence measurements the following data was obtained for protein X:
|
[GuHCl], M
|
Wavelength Maxima, nm
|
|
0
|
330
|
|
0.5
|
332
|
|
1.0
|
331
|
|
1.5
|
332
|
|
2.0
|
336
|
|
2.5
|
341
|
|
3.0
|
348
|
|
3.5
|
355
|
|
4.0
|
357
|
|
4.5
|
359
|
|
5.0
|
358
|
|
5.5
|
359
|
|
6.0
|
360
|
Use the above data to calculate the fraction of protein unfolded at each denaturant concentration. Plot the results to obtain a protein denaturation profile and calculate the transition mid-point of unfolding. What is the significance of the transition curve? What does the mid-point signify? What protein folding process does the data imply?