Discussion:
2-fold diltions
Perform a 2-fold serial dilution using 4 tubes containing 1ml of NB each and a 20ml broth culture of Bacillus subtilis; use the same B. subtilis culture and perform a 2-fold serial dilution using 4 tubes containing 2ml of NB each; repeat in a similar fashion using 4 tubes containing 0.5ml each.
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