Oxidation of glyceraldehyde-3-phosphate
Oxidation of glyceraldehyde-3-phosphate: The conversion of glceraldehyde- 3-phosphate to 1,3-bisphosphoglycerate is catalysed by glyceraldehyde- 3-phosphate dehydrogenase and the required cofactors are NAD' and Pi. The NADH formed is reoxidized either via the respiratory chain or by the NADH linked coilversion of pyruvate to lactate. The high-energy phosphate group at carbon 1 of 1,3-bisphosphoglycerate conserves much of the free energy produced by the oxidation of glyceraldehyde-3-phosphate. This is an example of substrate level pllosphorylation in which the production of a high-energy phosphate is coupled directly to the oxidation of a substrate instead of resulting-from oxidative plosphorylation via the election transport chain.