Using standard cloning technique, you have obtained a genomic actin clone from chicken using an actin probe from cornish game hen. when you use your chicken genomic actin clone against total cellular rna derived from a sample of skeletal muscle of chicken you find that two different rnas show strong hybridization to the chicken actin probe. after an extensive and exhaustive search against your chicken genomic library you are unable to obtain a second chicken genomic actin clone. the conclusion you reach is that the chicken genomic actin clone used as probe is hybridizing to two different sized mrnas which represent differentially spliced variants of a single genomic actin gene. you clone the two cdnas which represent the two mrnas and after sequence comparisons with each other and with the genomic clone you determine they are in fact two splice variants of a single gene. your next experiment is to quantitatively obtained from skeletal muscle at different stages of development. outline the experimental procedures