Problem:
DNA replication in bacteria is performed by the replisome molecular machine, which couples the activities of 11 different proteins in different enzymes.
Required:
Question 1: Name one enzyme utilized in DNA replication.
Question 2: Describe an experimental strategy based on degradation of specific proteins in the replisome to probe their functional importance.
Question 3: Briefly describe Slimfield fluorescence microscopy and its advantages for the study of molecular machines.
Could someone help to explain the answer of these questions.