Mode of inhibition. The kinetics of an enzyme are measured as a function of substrate concentration in the presence and absence of 2 mM inhibitor (I).
[S] (µM) Velocity (µmol/min)
No Inhibitor Inhibitor
3 10.4 4.1
5 14.5 6.4
10 22.5 11.3
30 33.8 22.6
90 40.5 33.8
a. What is the binding constant of this inhibitor?