Question 1
Match each of the following reagents with their purpose in the experiment:
1. Ethanol
2. Salt (in the DNA Extraction Solution)
3. Detergent (in the DNA Extraction Solution)
-Positive sodium ions bind with the negative phosphate charges on the DNA molecules to help the DNA molecules stick together and be more easily seen.
-Aids in breaking down the cell membranes to allow the DNA to leak out of the cell and into the solution.
-Helps separate carbohydrates and proteins from the DNA.
-Forces the DNA to precipitate out of the solution.
Question 2
Which of the following statements correctly describes the processes involved in the DNA extraction technique you used in this lab?
1.DNA is not soluble in either the ethanol or aqueous solution and so precipitates out.
2.DNA is soluble in the aqueous solution and the ethanol precipitates it out.
3.DNA is soluble in the ethanol causing it to precipitate out of the solution.
4.DNA is soluble in both ethanol and aqueous solutions and does not precipitate.
Question 3
The extracted DNA is a long strand with a sticky or gooey texture.
True
False
Question 4
An analysis of the extracted DNA would yield Adenine-Cytosine and Guanine-Thymine base pairs.
True
False