You ran the gel through Exercise 10 to look at the length of restriction fragments. You ran a gel throughout Exercise 12 to look at the length of amplicons. What determined the number of nucleotides in restriction fragments? What determined the number of nucleotides in amplicons?
What may have happened if you had employed D1S80 primers to amplify the segment of pLAF plasmid? What might have happened when you had digested your genomic DNA with
EcoRI and BpmI restriction enzymes?