1)In what order will the following proteins be eluted from anSP-sepharose column by an increasing salt gradient at pH 7: hemoglobin, serum albumin, lysozyme, pepsin, and fibrinogen?
2) In what order will the following proteins be eluted from aG-50 column: cytochrome c, myoglobin, pyruvate decarboxylase, hemoglobin, RNA polymerase, and titin ? Given your answer, whatmight be a more appropriate resin to use to separate this collection of proteins, and what would the elution order be with this more appropriate resin?