Hplc analysis of basic analytes is better to perform at low


HPLC analysis of basic analytes is better to perform at low pH when they are fully ionized (2 pH units lower than analyte pKa). For the analyte with pka= 5.6 and 60/40 acetonitrile/water eluent composition what should be the pH of an aqueous portion of the mobile phase to ensure complete ionization

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Chemistry: Hplc analysis of basic analytes is better to perform at low
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