How would you experimentally demonstrate using whole cells


You now have a putative beta5 receptor-expressing HEK cell line which binds [125I]-X100 specifically.

Discusswhat it means for the receptor to bind a ligandspecifically?

What are the features of specific binding?

How would you experimentally demonstrate, using whole cells, that X-100 specficially bindsthe beta 5 receptor expressed on the cell surface of these HEK cells? (note that this question is worth more than a few points and this is a molecular pharmacology course which requires that you leave the course understanding how binding is measured!!)

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