Problem: You need to seed cells into a 384-well plate at 2,000 viable cells/well in 50µl volume/well for a high-throughput small molecule library screen. You harvest the cells and end up with 16ml of medium with 1.20 million cells/ml. After testing for viability, you find that 90% of these are viable. Based on this information, please calculate the following. Show your calculations to receive full credit. Need Assignment Help?
a. Total number of cells in 16 ml =
b. Total viable cells in 16 ml =
c. Total viable cells in 1 ml =
d. How many Total number of viable cells are required to seed ONE 384 well plate
e. How many 384 well plates can you seed from the total viable cells that you have
f. How much of the stock cell suspension will you need to take and dilute to seed one 384 well plate at the required concentration (2000 cells/well) and volume (50 ul/well)? (N1= number of cells/ml). (NOTE: For this problem, you don't have to calculate extra volume to include pipetting error)