How many spots would light up in single gene of fish


Problem:

If you used a single gene as a probe for FISH on mitotic chromosomes, how many spots would light up?

A.One
B. Two
C. Three
D. Four

The answer is D, but why? The teacher said something about diploids and how we have mother and father contributions. I guessed it was 4 because there are 4 chromosomes on it and a single gene probe would light each one up. I guess people thought that meant only 2 would show up because they're sister chromosomes and therefore two duplicates?
Is anyone able to explain? I'm only choosing the best answer for an explanation, not just the answer. I have the answer.

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