1.) A 100 kDa protein is found to have a diffusioncoefficient value of 9.6 x 10^-7 in water at 37 cm^2/sdegrees C. Assuming a spherical shape, how many water molecules arebound by each protein?
2.) If you want to change the buffer composition of aprotein solution via diafiltration, how many resevoir volumes are required to remove 99% of the original buffer components?