Discussion:
a classic "dilution series" problem
Add 1ml bacteria to 99mls then 1m to 5 tubes of 9ml. Final and initial dilutions are required. Final dilution factor for plates are required. Plate one is from tube 3 adding 0.1 ml. Plate 2 is from tube 4 adding 1m. Plate 3 from tube 4 adding 0.1m. Plate 4 is adding 1ml ( 30 colonies are given for this plate) from tube 5. Plate 5 is adding 0.1 ml from tube 5. We are asked to give the expected number of colonies for plates 1,2,3,5. We are asked how many microbes/ml are in orginal sample of bacteria.
I know 99 ml + 1ml is 1/100 or 10 negative 2 and is the intial and final dilution for 99 ml tube, but I have had no clear instruction on the step by step procedure of the problem! Please give clear and simple instructions so I may solve this diluton problem!