Problem:
How can I improve my Gel Extraction yields. We use the standard protocol from Qiagen, gel extraction, dissolve in QG buffer at 42C and purify via anion exchange columns.
However, with 500 ng we ultimately get 100 ng for a 20% yield. I was curious what else has been tried for gel extraction.
We also add IPA and NaOAc to the dissolved mixture prior to the column purification. Please provide some suggestion.