Problem:
Question 1: How can mutations accumulate, given that cells have repair systems that fix changes or breaks in DNA strands? Please explain in detail.
Question 2: The product of the phosphatase reaction you will be studying is expected to reach a maximum of ~150 µM in your studies. Assume this product is either a monoacidic or monobasic product. If you make the 10 mM buffer in part with a volume of 1 L, will the buffer be strong enough to keep the change in solution pH to less than 0.1 pH unit?
Justify your answer with calculations. The buffer used is Tris and I assumed it to be 1 M concentration in stock solution. The Diliution had to be 10 mM and 1 L. Show the pedigree and your calculation.