How bile esculin agar is prepared


Problem:

Bile Esculin Test

Sometimes Bile Esculin Agar is prepared as slants. With the form of the medium, only organisms able to darken more than half of the agar within 48 hours are considered positive (in contrast to the plated medium where any darkening is positive).

Required:

Question: Why do you think this is so?

Hint: Consider the designated purpose of agar slants and plates.

My guess: less area in the plate?

Does anyone know how to go about this search to explain it.

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Biology: How bile esculin agar is prepared
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