1. How are microsatellite markers scored (what are the alleles)?
2. Why are microsatellites codominant markers?
3. What are the advantages and disadvantages of using RFLP, AFLP, and microsatellite markers? If you don’t know any sequence information for the organism, which marker do you want to use?
4. How does the modern Sanger sequencing technique work? What is the purpose of the ddNTPs? How is the order of nucleotides determined?
5. What does a sequencing reaction contain?
6. How does a cloning vector allow us to sequence an unknown segment of DNA?
7. What are the advantages/disadvantages to Roche 454 Pyrosequencing and Solexa Illumina sequencing over the Sanger method?
8. Which method was used to generate the first human genome? How much did it cost?
9. How much did it cost to sequence the whole human genome using the HeliScope Sequencing System?