How are benign and malignant tumors different? How is a diagnosis of benign versus malignant made for a solid tumor? What characteristics of the tumor are most commonly assessed when the diagnosis is made?
Describe the technique for making transgenic mice by pronuclear injection of DNA.
What are some of the uses for making transgenic mice by this method? How can this technique be used to study the role of gene expression in development and disease?
Describe one approach for using this technique to diminish the function of a gene or its product.
Describe the two enabling hallmarks of cancer. How, mechanistically speaking, do we think they contribute to carcinogenesis?