Fluorescence in situ hybridization (FISH) is a technique wherechromosomes on a slide can be denatured, allowing single-strandedDNA probes labelled with a fluorescent dye to anneal to genomicDNA.
For each of the following fluorescent probe DNAs, draw a humanchromosome in metaphase and indicate how the chromosome will bestained by hybridization of the probe:
- a single copy gene
- rDNA (ribosomal RNA genes, assume it is one of the 5 humanchromosomes with rDNA)
- a high copy transposon dispersed throughout the genome
- telomeric DNA
- very high copy satellite DNA with a repeat unit of about 180bp