For homework, I had to prove that a certain catalyst was a protein, then tell how I would purify the protein, then tell how I would clone a human chromosomal region that contains the gene encoding the protein. The last part of the question is where I am stuck which says: how would you verify that one of the genes you isolated does indeed encode a protein with the catalytic properties of the protein being studied?
Can someone please give me a good, detailed explanation as to an experiment I could do to verify that the gene I cloned encodes the protein I am interested in?