Enzymes explained in this answer
Would you help me with the following questions?
An enzyme is found that catalyzes the reaction Aï??B. Researchers find that the Km for the substrate A is 4 uM, and the kcat is 20 min-1.
a. In an experiment, [A]=6 mM, and the initial velocity, V0, was 480 nM min -1. What was the [Et] used in the experiment?
b. In another experiment, [Et] = 0.5 uM, and the measured V0 = 5 uM
min-1. What was the [A] used in the experiment?