Explain what is affinity chromatography explain how does a, Chemistry

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Explain what is affinity chromatography explain how does a

1. Explain what is affinity chromatography?

2. Explain how does a protein a column work in the purification of an antibody?

3. Explain what is the need for equilibration of a protein A column with the binding buffer?

4. Explain what will happen if you do not wash the column before elution?

5. Define what is the principle behind elution of an antibody from a protein a column?

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