Explain the Principle of Nelson-Somogyi Method?
Glucose is estimated by Nelson-Somogyi method. We begin our study of this method by getting to know the principle involved in this estimation.
Principle
All sugars which contain a free aldehyde group undergo enolization when placed in hot alkaline solution. The enediol form of sugars is highly reactive and is easily oxidized by oxidizing agents in alkaline solution. This property is utilized in the quantitative determination of sugars. The sugar solution is heated with alkaline copper reagent in a Folin's tube. The solution is made to react with arsenomolybdate solution to produce a blue colour. The intensity of this colour is measured colorimetrically.