Explain the Plating Method - Pure Culture Techniques?
Plating method is one of the commonly employed methods for getting a pure culture. The procedure involves the separation and immobilization of individual organisms on or within the nutrient agar medium. Each viable cell then multiplies and produces an isolated colony - a visible mass of microbial cells on solid surface, which is obtained by multiplication of a single organism. These colonies can then be transferred readily to nutrient broth or nutrient agar slant to get a pure culture.
There are different methods of plating, which are used for getting a pure culture. These include -
1. Streak Plate Method,
2. Spread Plate Method, and
3. Pour Plate Method.