Explain the Composition of Sabouraud Agar?
Sabouraud agar is used for cultivation of yeasts and moulds.
Peptone - 10.0 gm
Dextrose - 40.0 gm
Agar - 15.0 gm
Distilled water - 1000 ml
pH - 5.6
Dissolve all the ingredients except agar in distilled water. Adjust the pH to 5.6. Add the agar and dissolve by heating. Dispense the media into flasks and autoclave. For preparing Sabouraud agar supplements with aureomycin, aseptically add 10 µg/ml of aureomycin to the sterile molten and cooled media.