Explain the Composition of Blood Agar?
Infusion from beef heart - 500 gm
Tryptose - 10.0 gm
Sodium Chloride - 5.0 gm
Agar - 15 gm
Distilled water - 1000 ml
pH - 7.3
Dissolve above ingredients and autoclave. Cool to 45-50°C and add 50 ml of sterile defibrinated blood aseptically. Rotate to mix thoroughly and pour immediately into sterile plates or tubes before solidification.