Explain Procedure for Sub-Culturing of a Culture
Now carry out the exercise following the steps given herewith:
1. Sterilize the inoculating loop and needle by heating to red hot.
2. Label all the tubes and nutrient agar plates.
3. Following the protocol given in section 4.3, transfer the E. coli and Bacillus sp. culture to:
(a) Nutrient agar tube,
(b) Nutrient slant and deep tube, and
(c) Nutrient broth.
4. Incubate the culture at 37°C and observe the growth after 24 hours.