Explain Procedure for Simple Staining of Bacterial Cultures?
Now carry out the exercise following the steps given herewith:
(1) Prepare thin bacterial smear on clean glass slide.
(2) Fix the bacterial smear on glass slide by heat fixation.
(3) Place the slide on the staining tray.
(4) Flood the smear with a staining reagent for appropriate exposure time.
(5) Remove the excess stain by gentle washing with tap water. The slide should be held parallel to the stream of water to avoid removal of microorganisms from slide.
(6) Blot dry the slide.
(7) Examine the dried slide under microscope in oil immersion (100x). First fix the slide at 10x and then move to 100x. (Oil immersion).