Problem:
Question 1: What are 2 ways in which the wound assay is prone to mis/over interpretation when used as a model for cancer cell invasion?
Question 2: Explain the effects of treating the wounded cultures (when performing a wound assay) with the added inhibitor (Cytochalasin B). What are the targets of this inhibitor and what would inclusion of these inhibitors tell you about mechanisms supporting cell movement? (3-4 sentences)
Any help would be greatly appreciated!