Examining dna plasmid restriction digest


Discussion:

Examining DNA plasmid restriction digest

These questions ask:

1. How do you predict the DNA bands produced after digesting a recombinant plasmid. How do you know what size your recombinant plasmid is after cloning and ligation of the insert into the plasmid.

2. How do you prepare a master mix to use for a restriction digest.

3. What is a plasmid copy number and how do you estimate the copy number for a plasmid

Write your answer/response in detail with examples and facts and figures using APA style of formatting, size 12, font Times new roman and answer must be single spaced

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