estimation of nitrogen and protein content using

Question

Estimation of Nitrogen and Protein Content Using Micro Kjeldahl's Method?

Standardization of H₂SO₄

Prepare a solution of 0.1 N H₂SO₄  by diluting 2.8 ml of the concentrated acid to 1000 ml in a volumetric flask. This is approximately 0.1N. Standardize the acid against 0.1 N Na₂CO₃ solution. Accurately weigh 0.530 g of anhydrous Na₂CO₃. Transfer to a 100 ml volumetric flask and make up the volume to the mark with distilled water.

Pipette 10 ml of this solution in a conical flask and titrate against the diluted H₂SO₄ prepared using 1% solution of methyl orange as an indicator. Calculate the exact normality of H₂SO₄ and dilute it accordingly to obtain exactly 0.0143N H₂SO₄.