Problem: Previously, to learn about the effects of different genes, the gene was knocked out before development and in every cell in the organism. What were the issues with this technique? How does the TRiP approach improve over how gene knockouts were done before?
End reference: Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2002). Studying Gene Expression and Function. Molecular Biology of the Cell. 4th Edition.
What is the purpose of the insertion controls used in this experiment?
End reference: Chandler, M., & Siguier, P. (2013). Insertion Sequences. In S. Maloy & K. Hughes (Eds.), Brenner's Encyclopedia of Genetics (Second Edition) (pp. 86-94). Academic Press.\