Describe how you would prepare 1.000 L of 0.05M HEPES buffer @ pH 7.45 in the lab. You have pure HEPES, a pH meter, 1.00 M HCl and 1.00 M NaOH, and all of the pure water and glassware you need. Carefully go through all the mathematical steps, and then describe how you would make the buffer in the laboratory.