Problem:
Please answer both the questions in detail:
Question: Do you need a transducer to process the signal coming from a electrode impaling a crayfish muscle? Why or why not?
Question: What kinds of detergent-free bacterial lysis buffers exist? The proteins we're extracting will be later analyzed by LC-MS/MS, and we're looking for a lysis buffer that won't interfere with this downstream analysis.
Any help would be greatly appreciated!