Problem:'
Instead of using polymerase Vent exo (-), can I go with the usual Taq polymerase?
Question: Do the PCR conditions change (the temperature and master mix concentrations ) in the these two conditions?
Question: Do the primers (sequence and concentrations) change if we use vent exo instead of Taq polymerase and vice versa?
Question: If the primers are designed specifically for Vent exo (-) how can I modify the PCR conditions so that I can use the same primers (meant for vent exo) using Taq polymerase? Justify your answer.