Problem: Finally you found time to take a lunch break. While in the break room, Monte comes in and asks for immediate help. He states that he received a spinal fluid specimen on an infant from emergency department. He accidently spilled almost all of the specimen! It is imperative a culture is performed without having to collect another specimen. After examination, you discover there are 2 drops remaining in the spinal fluid tube. The procedure for culturing a spinal fluid requires a gram stain, blood, macconkey, chocolate, and thioglycollate broth to be set up. Forget about your lunch! Think about the types of organisms that will grow in each of the media types, and the importance of the gram stain. What would you recommend to do with the 2 drops? Why is a gram stain crucial in a spinal fluid specimen? How is the spinal fluid gram stain processed and prepared under normal circumstances when there is plenty of specimen? Colony Morphology What type of media is used to detect hemolysis? Explain the appearance of beta, alpha, and gamma hemolysis. What type of media is used to detect lactose fermentation? What type of media is commonly used to grow fastidious bacteria? What organism is known to "swarm?" What organism is known to be very "mucoid?" Staph aureus is inoculated and incubated on blood, macconkey, and chocolate agar. If the bacteria grow on the blood and chocolate agar, but not macconkey, what type of bacteria is it? Need Assignment Help?