Discuss the protein concentration of the undiluted


Aliquots of a 0.5 mg/ml standard of BSA are used to construct a standard curve for the bradford protein assay. The tubes contains the following amounts of the BSA solution: 0,20,40,60,80, and 100μL. The cooresponding absorbencies after adding bradford reagent are 0, .05, .09, .14,.19, and .22. If you take 20μL of an unknown and add 80μL of water, mix, take 10μL of the mixture and add to bradford reagent and see an ansorbance of .08 what is the protein concentration of the undiluted unknown?

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Chemistry: Discuss the protein concentration of the undiluted
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