Discription on an optimization of potential pitfalls, Biology

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Discription on an optimization of potential pitfalls

Problem:

Has anyone optimized RT for long transcripts (9kb)? The downstream application will be PCR amplification and Illumina library prep. It will be trivial to make internal primers sets for the PCR that are specific as long as there are no chimeric sequences. If there are, they will probably get primed also. If anyone knows of an optimization and/or other potential pitfalls, I would love to hear them.

Please anyone could provide more description about it.

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Biology: Discription on an optimization of potential pitfalls

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Biology: Discription on an optimization of potential pitfalls

Reference No:- TGS0876444

Have a Question? (oR Write a Review)

Recent Questions Asked Biology

Q : Are dark marks aka moles on our skin a patch of dead cells

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Biology: Discription on an optimization of potential pitfalls

Reference No:- TGS0876444

Recent Questions Asked Biology

Q : Are dark marks aka moles on our skin a patch of dead cells

Q : How do the ion transporters

Q : Explain some eaten inevery culture today

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