Assignment:
A spectrophotometric assay was to be carried out on a compound ε = 5000. The stock solution of standard had been made with a concentration of 0.0203M. Given a 1.000cm path length and a desired absorbance around 0.4-0.5, by what factor should the stock solution be diluted?
- Exactly how would you best perform this dilution in the lab?
Provide complete and step by step solution for the question and show calculations and use formulas.