1) You ran a gel during Exercise 10 to look at the length of restriction fragments. You ran a gel during Exercise 12 to look at the length of amplicons. What determined the number of nucleotides in the restriction fragments? What determined the number of nucleotides in the amplicons?
2) What might have happened if you had used D1S80 primers to amplify a segment of the pLAF plasmid? What might have happened if you had digested your genomic DNA with the EcoRI and BpmI restriction enzymes?