Differentiation of cells is not easily reversible, Biology

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Differentiation of cells is not easily reversible

Differentiation of cells is not easily reversible because it involves which one of the following?
1. changes in the nucleotide sequence of genes within the genome.
2. frameshift mutations and inversions.
3. excision of some coding sequences.
4. chemical modifications of histones and DNA methylation.
5. changes in chromatin structure that make certain regions of the genome more accessible.

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