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Determine the max binding constant for the protein complex


Discussion:

Plot the following data using a Sacatchard type plot. The change in fluorscence is proportional to binding.

A) Determine the max binding constant for the protein complex.

Realtive Fluorescence (Response) [Protein] nM (concentration of added protein)

  • 5.70E+04 | 0
  • 5.73E+04 | 0.1
  • 5.78E+04 | 0.3
  • 5.89E+04 | 1
  • 6.03E+04 | 3
  • 6.13E+04 | 10
  • 6.17E+04 | 30
  • 6.18E+04 | 50

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Biology: Determine the max binding constant for the protein complex
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