Discussion:
Plot a Lineweaver-Burk problem
A) Using the data given in table below plot a Lineweaver-Burk plot
b) Determine the Km and Vmax for the three sets of data
c) Which inhibitor would the most effective at high substrate concentrations?
d) Explain you choice
Table 1 :
Kinetics data for an enzyme catalysed reaction with and without the presence of inhibitors :
[S] mmol/L : 1.67 - 2.50 - 5.00 - 10.00
Initial velocity of reaction (micromol/min) :
without inhibitior : 0.100 - 0.125 - 0.167 - 0.200
with inhibitor A : 0.083 - 0.106 - 0.149 - 0.185
with inhibitor B : 0.068 - 0.083 - 0.105 - 0.122