1. Determine the amino acid sequence from the following data.
Composition: 3 Ala, Arg, Asp, Gly, His, Leu, 2 Lys, 2 Met, Thr, Trp, Tyr, Val
Edman: Ala-
Tryptic peptides:
Tp-1: Ala, Asp, Lys, Trp
Edman: Ala-
Tp-2: Ala, Arg, Gly, Met, Thr
Edman: na
Tp-3: Ala, His, Leu, Lys, Met, Tyr, Val
Edman: na
Chymotryptic peptides:
Ch-1: Ala, Asp, Trp
Edman: Ala-
Ch-2: His, Leu, Lys, Met, Tyr, Val
Edman: na
Ch-3: 2 Ala, Arg, Gly, Lys, Met, Thr
Edman: na
CNBr peptides:
C-1: Ala, Arg, Gly
Edman: Ala-
C-2: Ala, Asp, His, Homoser lactone, Lys, Trp
Edman: Ala-
C-3: Ala, Homoser lactone, Leu, Lys, Thr, Tyr, Val
Edmn: Leu-
2. Determine the amino acid sequence from the data.
Composition: Ala, Arg, Asp, 2 Gly, Ile, 2 Leu, 2 Lys, Met, Phe, Ser, Thr, Tyr, Val
Edman on whole peptide: Gly-
Tryptic peptides:
Tp-1: Composition: Ala, Gly, Lys, Tyr
Edman Gly-
Tp-2: Composition: Arg, Gly, Leu, Ser, Thr
Edman: Gly-Thr-Leu-
Tp-3: Composition: Asp, Ile, Leu, Lys, Met, Phe, Val
Edman: Phe-
Chymotryptic peptides:
Ch-1: Composition: Gly, Tyr
Ch-2: Composition: Asp, Ile, Leu, Lys, Met, Val
Edman: Asp-
Ch-3: Composition: Ala, Arg, Gly, Leu, Lys, Phe, Ser, Thr
Edman: Ala-
CNBr peptides:
CNBr-1: Composition: Ile, Leu, Lys, Val
Edman: Val-Leu-
CNBr-2: Composition: Ala, Arg, Asp, 2 Gly, Leu, Lys, Homoser lactone, Phe, Ser, Thr, Tyr
Edman: Gly-Tyr-