Question - You work in a government lab and are given two cultures of a particular bacterium. The bacteria in culture A can grow on minimal medium. Culture B is a derivative of that parent strain and culture B cannot grow on minimal medium but can grow on minimal medium that has the amino acid threonine added.
What would you call strain B?
You have been tasked with setting up an experiment to determine if a new human drug is mutagenic and therefore likely to be a carcinogen. You decide to determine whether this new drug increases mutation rates. Given that you must test this using either, or both, of the strains A and B described above, describe how you would set up such an experiment and the theory behind it. Include all necessary steps and a discussion of controls and data interpretation.