The protocol tells you to make the given dilution:
Label 5 tubes 1 to 5. Add 1.50 mL of diluent to every tube. To first tube (#1) add 500 uL of 1.00 M glucose and mix. Transfer 500 uL from the first tube to second (#2) and mix. Like wise, continue to transfer 500 ul from every tube of remaining tubes to tube which follows it until all 5 tubes have been utilized. Determine final concentrtion of glucose in tube 5?