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Describe the datsenko and wanner method of gene disruption

Describe the Datsenko and Wanner method of gene disruption in E.coli. Describe how you will approach the problem of knocking two genes out in the same strain if

a. The two genes are part of the same operon

b. The genes are very far part in genome location

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Biology: Describe the datsenko and wanner method of gene disruption

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